Coding

Part:BBa_K3301002:Design

Designed by: Maria Lyons, Hailey Ho, Vina Chen, Zoe Hsieh, Josh Dayie   Group: iGEM19_Baltimore_BioCrew   (2019-10-15)


Codon optimized B. theta gene BT2269


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 998
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed initial signal sequence. Codon optimized for E. coli.


Source

Synthesized by IDT

References